The objective of this work was to obtain buffalo milk powder through the process of drying in mini spray dryer, and evaluate its physical, chemical, structural and proteomic features during storage. The buffalo milk was subjected to drying by atomization, air temperature of 140°C inlet. The samples were evaluated at times 0, 30, 60, 90, 120 and 150 days of storage. The moisture content, fat, protein, ash, density, acidity, solubility index, burning particles were analysed, along with structural analysis by Scanning Electron Microscopy (SEM) and proteomic study (SDS-PAGE). The production of milk powder from the set standards produced a buffalo milk powder with satisfactory results of physical and chemical compositions. In the SEM analysis was possible to observe an accumulation of milk powder particles with withered and wrinkled structures. Variations in the intensity of the bands of protein fractions were observed by electrophoresis, but no significant changes. The production secured buffalo milk powder with good quality characteristics, without any undesirable changes in the product during the storage time.